Advances in genome engineering have resulted in the generation of numerous zebrafish mutant lines.A commonly used method to assess gene expression in the mutants is in situ hybridisation (ISH).Because the embryos can be distinguished by genotype after ISH, comparing gene expression between wild-type and mutant siblings can be done blinded and in pa
BACKGROUND: The phenotype of large diameter sensory afferent neurons changes in several models of neuropathic pain.We asked if similar changes also occur in "functional" pain syndromes.METHODOLOGY/PRINCIPAL FINDINGS: Acidic saline (AS, pH 4.0) injections into the masseter muscle were used to induce persistent myalgia.Controls received saline at pH
ABSTRACTMosquito saliva facilitates blood meal acquisition through pharmacologically terracotta charger active compounds that prevent host hemostasis and immune responses.Here, we generated two knockout (KO) mosquito lines by CRISPR/Cas9 to functionally characterize D7L1 and D7L2, two abundantly expressed salivary proteins from the yellow fever mos